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Acetyl-Histone H3.1(...

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Acetyl-Histone H3.1(K56)Antibody

  恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。

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Acetyl-Histone H3.1(K56)Antibody

 
  • 貨號:
    HYK-200281
  • 規格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min
      All lanes:Acetyl-Histone H3.1(K56)antibody at 0.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 15 KDa
      Observed band size: 15 KDa
    • Immunocytochemistry analysis of CSB-RA010418A56acHU diluted at 1:100 and staining in HepG2 cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010418A56acHU at 1:43,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

產品詳情

  • Uniprot No.:
    P68431
  • 基因名:
    HIST1H3A
  • 別名:
    H3 histone family member E pseudogene antibody; H3 histone family; member A antibody; H3/A antibody; H31_HUMAN antibody; H3F3 antibody; H3FA antibody; Hist1h3a antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody; HIST1H3E antibody; HIST1H3F antibody; HIST1H3G antibody; HIST1H3H antibody; HIST1H3I antibody; HIST1H3J antibody; HIST3H3 antibody; histone 1; H3a antibody; Histone cluster 1; H3a antibody; Histone H3 3 pseudogene antibody; Histone H3.1 antibody; Histone H3/a antibody; Histone H3/b antibody; Histone H3/c antibody; Histone H3/d antibody; Histone H3/f antibody; Histone H3/h antibody; Histone H3/i antibody; Histone H3/j antibody; Histone H3/k antibody; Histone H3/l antibody
  • 反應種屬:
    Human
  • 免疫原:
    A synthesized peptide
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, ICC, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    ICC 1:50-1:500
    IF 1:30-1:200
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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