歡迎選擇我們，上海恒遠生物科技有限公司！免費服務熱線：400-0899-208 防偽查詢

語言：Eng

您當前的位置：: 首頁>; 產品中心>; 抗體>; 重組抗體>; SARS-CoV-2 Spike RBD...

重組抗體

SARS-CoV-2 Spike RBD Nanobody

　　恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗)，包括人類常用抗體，模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。

　　恒遠生物擁有專業的質控團隊，配備先進實驗儀器，現已成功地建立了ChIP平臺，因此我們的抗體不僅能提供Biotin、FITC、HRP等標記，大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。

　　我們致力于為所有用戶提供高親和力、高特異性的優質抗體，歡迎來電咨詢。

產品詳情相關產品

SARS-CoV-2 Spike RBD Nanobody

貨號：

HYK-200093
規格：
圖片：
The Binding Activity of SARS-CoV-2 Spike RBD Nanobody with SARS-CoV-2-S1-RBD
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD () at 2 μg/ml can bind SARS-CoV-2 Spike RBD Nanobody, the EC50 is 0.8674 ng/ml.

In the Colloidal Gold Immunochromatography Assay
detection system, the background of antibody (CSB-RA33245A2GMY) is clean, the detection limit can be as low as 25ng/ml (1.75ng/0.07ml), and the sensitivity is very good.

Binding signal of SARS-CoV-2-S1-RBD () and ACE2 protein-HRP conjugate () was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the ICC50 is 1.296 nM.

Binding signal of SARS-CoV-2-S1-RBD () and ACE2 protein-HRP conjugate () was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/ml.

SARS-CoV-2 Spike protein RBD His/Sumostar Tag () captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay.

產品詳情

產品描述：

This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) antibody is a recombinant monoclonal antibody (also a Nanobody) generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA).
It is also validated in Neutralizing and LSPR. In neu assay, the binding signal of the SARS-CoV-2 S1 RBD antibody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/ml IC50. In LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM).
Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.
Uniprot No.：

P0DTC2
別名：

Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov S1 Antibody;Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus S1 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-Spike RBD Antibody; E2 Antibody; E2 glycoprotein Antibody; Human coronavirus spike glycoprotein Antibody; S Antibody; SARS-CoV-2 S1 RBD Antibody; S glycoprotein Antibody; Spike glycoprotein Antibody
反應種屬：

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
免疫原：

Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
免疫原種屬：

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

VHH fusion with human IgG1 Fc
純化方式：

Affinity-chromatography
克隆號：

A1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, GICA, Neutralising

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:10000-1:100000
GICA	1:10000-1:40000
Neutralising	1:100-1:10000

Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

N Antibody

SARS-CoV-2 Spike RBD Nanobody, HRP conjugated

SARS-CoV-2 Spike RBD Nanobody, FITC conjugated

SARS-CoV-2 Spike RBD Nanobody, Biotin conjugated

Phospho-Histone H3.3 (T3) Antibody

Di-methyl-Histone H3.1(K4)Antibody

在線詢價

*
*
*
*
留言內容 *
*

上一篇：N Antibody: 下一篇：S Antibody, Biotin conjugated

組織胺H4受體抗體

恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗)，包括人類常用抗體，模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。恒遠生物擁有專業的質控團隊，配備先進實驗儀器，現已成功地建立了ChIP平臺，因此我們的抗體不僅能提供Biotin、FITC、HRP等標記，大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。我們致力于為所有用戶提供高親和力、高特異性的優質抗體，歡迎來電咨詢。

查看詳細